Fig. 3: LSEC micrographs.
From: Label-free superior contrast with c-band ultra-violet extinction microscopy
a–c Label-free imaging of fixed LSECs using visible light brightfield (BF), differential interference contrast (DIC), quantitative holotomography (HT), and using our UV microscope (d, e) showing either a (d) raw UV intensity image or (e) quantitative UV extinction (qUV). (f, g) Fluorescence imaging of membrane-labeled LSECs using widefield (WF) and superresolution structured illumination microscopy (SIM). h 2.5x enlarged views of similar regions. The scale bars are 5 µm in (a–g) and 1 µm in (i). Label-free images were computationally adjusted with unsharp masking for enhanced visibility of the sieve plate. Enlarged views of panels d and e are shown in Supplementary Fig. S4
