Fig. 5: Analysis of fenestration clusters.
From: Label-free superior contrast with c-band ultra-violet extinction microscopy
a UVC LSEC image. b Image after qDPC processing. c Segmentation of the plasma membrane using a Weka classifier25. d–f Separation of plasma membrane (blue) from fenestration clusters (orange) using a sliding paraboloid filter. g Enlarged view of (a). h, i Enlarged views of (a) and (d) with marked fenestration clusters. j Histogram of detected fenestration cluster extinction coefficients from (f) in yellow and extinction coefficient histogram in blue of the segmented plasma membrane in (e). The scale bars are 5 µm
