Fig. 5: Semi-quantitative assessment of high-RI structures using in-silico clearing RI tomography. | Light: Science & Applications

Fig. 5: Semi-quantitative assessment of high-RI structures using in-silico clearing RI tomography.

From: In-silico clearing approach for deep refractive index tomography by partial reconstruction and wave-backpropagation

Fig. 5

a Maximum intensity projection (MIP) and b cross-sections of the RI maps of HepG2 spheroids at oleic acid (OA) concentrations of 0, 75, 150, and 300 µM. The depth of cross-sections is identified with white dashed lines in MIP images. c 3D rendering of RI distribution at OA concentrations of 0 µM and 300 µM, respectively. Plots of (d) spheroid volume, (e) equivalent diameter, and (f) volume ratio of high-RI structures as a function of OA concentration. Six spheroids are measured for each OA concentration (a total of 24 spheroids). The fitted line in (f) has a slope of 0.0069%/µM and an intercept of 0.23% with a coefficient of determination R2 = 0.90

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