Fig. 6: Semi-quantitative assessment of staurosporine-induced morphological changes in HepG2 spheroids using in-silico clearing RI tomography. | Light: Science & Applications

Fig. 6: Semi-quantitative assessment of staurosporine-induced morphological changes in HepG2 spheroids using in-silico clearing RI tomography.

From: In-silico clearing approach for deep refractive index tomography by partial reconstruction and wave-backpropagation

Fig. 6

a Maximum intensity projection (MIP), b cross-sections, and c cross-sections merged with non-fragmented/fragmented areas of RI maps of HepG2 spheroids at staurosporine concentrations of 0, 300, and 5000 nM, respectively. The cross-section is identified with white dashed lines in the MIP image. The movies showing all cross-sections are provided in Supplementary Movies 4–6. Plots of (d) spheroid volume, (e) equivalent diameter, and (f) non-fragmented ratio as a function of staurosporine concentration. Six spheroids were measured for each staurosporine concentration (a total of 72 spheroids). The black solid line in (f) denotes the fitted based on the Hill model. The relative 50% effective concentration EC50 = 233 nM and the Hill exponent H = 1.71

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