Fig. 3: Super-resolution images from localization microscopies evaluated by rFRC. | Light: Science & Applications

Fig. 3: Super-resolution images from localization microscopies evaluated by rFRC.

From: Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlation

Fig. 3

a STORM result of α-tubulin labeled with Alexa Fluor 647 in a COS-7 cell (left). b rFRC map of (a). c The merged view of STORM result (green hot) and Gaussian averaged rFRC map (shifted jet) for highlighting low-quality regions. d 3D-STORM results (COS-7 cells, α-tubulin labeled with Alexa Fluor 647). e 3D rFRC map of (d). f PANEL after the Otsu threshold of (e). g Corresponding magnified horizontal sections of the 3D-STORM (left) and rFRC (right) volume of the white boxes in (d). h The curve of the rFRC values along with the axial positions. Scale bars: (a, d) 5 μm; (g) 2 μm

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