Fig. 6: Diverse optical imaging approaches assisted and evaluated by the rFRC map. | Light: Science & Applications

Fig. 6: Diverse optical imaging approaches assisted and evaluated by the rFRC map.

From: Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlation

Fig. 6

a Representative images of live human umbilical vein endothelial cells (HUVECs) labeled with LifeAct-EGFP under Wiener-SIM (top) and TIRF (bottom) imaging. b Hessian-SIM result. c rFRC map of Hessian-SIM. The rFRC, RSP, and RSE values of Wiener-SIM (magenta) and Hessian-SIM (cyan) are shown on the bottom right. d Representative results of fixed liver sinusoidal endothelial cells (LSECs) labeled with DiI under RL deconvolution (top) and TIRF (bottom) imaging. e rFRC map of RL deconvolution result. f Magnified views of the white box in (d). The original TIRF image, RL deconvolution results with 80 and 200 iterations, and TIRF-SIM results are shown in the top left, top right, bottom left, and bottom right, respectively. g Curves of the PSNR (versus TIRF-SIM), RSP (versus TIRF), and rFRC values over iterations. h Simulated ground-truth. i Wide-field image of (h). j Corresponding Fourier ptychographic microscopy (FPM) reconstruction. k RSM of FPM. l rFRC map of FPM. m Merged image of PANEL (green channel) and FPM (gray channel) results. Cyan and magenta arrows represent the false positives and the true negatives, respectively. Scale bars: (a) 1 μm; (d) 5 μm; (f) 100 nm; (j) 50 μm

Back to article page