Fig. 3

Axial tracking of single particles via DH-PSF iSCAT microscopy. a–c iSCAT images of the same particle at three different axial positions (Z-axis), (d) typical calibration curve of the angle between two lobes on (a–c) with respect to the axial position measured with a piezo-controlled stage. e–h Simulated DH-PSF iSCAT images and the corresponding calibration curve. Scale bars on (a, e), 1 µm