Fig. 6
Simulated PSF images of standard iSCAT microscopy and DH-PSF iSCAT microscopy. a1–a6 Standard PSF image of iSCAT working in reflection mode; (b1–b6) standard PSF image of iSCAT working in transmission mode (also named COBRI); (c1–c6) DH-PSF iSCAT image. The imaged object is a single polystyrene particle with a diameter of 100 nm. The three rows of images are recorded when this single nanoparticle moves axially within a range of 1 μm near the DOF of the imaging objective lens (100 X, NA 1.49). The axial distance traveled by the nanoparticle on adjacent images is 200 nm. Scale bar, 1 µm. The right color bars denote the imaging contrast, whose definitions are given in the Materials and methods section
