Fig. 10: Images of a 50μm thick Thy-1 -GFP M line transgenic mouse brain slice in both line scanning off-axis separated detection and wide-field coaxial systems to compare SIM and HiLo in different detection approaches.
From: Optical sectioning methods in three-dimensional bioimaging
a, b Maximum intensity projections (MIPs) of the mouse brain slice by SIM reconstruction via wide-field (WF) and line scanning imaging approaches, respectively. c, d WF-SIM- and WF-HiLo-reconstructed enlarged views from the red square in a, respectively. e, f DSIM- and DHiLo-reconstructed enlarged views of the red square in (b), respectively. g, h Enlarged images from the yellow squares in (c) and (e), respectively. i, j Enlarged images from the blue square in (c) and (e), respectively. k, l Enlarged images from the yellow square in (d) and (f), respectively. m, n Enlarged images from the blue square in (d) and (f), respectively. Scale bar: 1 mm in a and b, 100 μm in c–f, and 10 μm in g–n
