Fig. 3: 3D z-stack images of lysosomes and microtubules. | Light: Science & Applications

Fig. 3: 3D z-stack images of lysosomes and microtubules.

From: Phasor-FSTM: a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing

Fig. 3: 3D z-stack images of lysosomes and microtubules.

a Unresolved confocal images at different imaging depths (z-step: 0.5 μm) in a HeLa cell. b 3D z-stack of confocal images in panel a before unmixing (different colors indicate different depths). c Fluorescence decay curves of the original FLIM data. d Phasor plots change with the imaging depth. e Comparison of two-color confocal and Phasor-FSTM in terms of 3D z-stack images. SBRs were calculated to demonstrate the improvement in image quality of the proposed method compared to confocal. f Normalized intensity profiles along the arrows in the enlarged images of e. g Mean FWHM values of the intensity profiles across the structures at five positions. Scale bars, 5 μm (a, b, e)

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