Fig. 4: Phasor-FSTM for four-color super-resolution imaging in live cells. | Light: Science & Applications

Fig. 4: Phasor-FSTM for four-color super-resolution imaging in live cells.

From: Phasor-FSTM: a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing

Fig. 4: Phasor-FSTM for four-color super-resolution imaging in live cells.

a Confocal image of a single HeLa cell consisting of the mitochondrion (MitoTracker Deep Red FM), microtubule (Tubulin Tracker Deep Red), lysosome (LysoBrite NIR), and nucleus (Nuclear Red LCS1) structures. b FSTM image without the phasor process. c Four-color phasor-FSTM super-resolution images before (top right) and after (lower left) contrast adjustment. Mitochondrion: yellow; microtubule: green; lysosome: magenta; nucleus: cyan. d Magnified views of the white squares in a–c. e Photon number distributions in original diffraction-limited and super-resolution images, and intensity profiles in one-color and four-color super-resolution images post-deconvolution, plotted along the dotted lines in d

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