Table 2 Fluorescent probes used in live microscopy of organoids
Probe | Physiological parameter | Stock concentration (Solvent) | Staining concentration | Excitation/Emission (nm) | Fluorescence Lifetime (τ, ns), [citation] |
|---|---|---|---|---|---|
AF488-WGA | Binds N-acetyl-D-glucosamine and sialic acid; mucous membranes72 | 1 mg/mL (PBS) | 4 μg/mL | 490/495 - 520 | 1.2-2.6 ns [this study] |
Fluorescein-UAE-1 | Glycoproteins, glycolipids with α-fucose residues73 | 5 mg/mL (PBS) | 5 μg/mL | 490/495 - 520 | NA (No staining observed) |
Nile Red | Solvatochromic dye. Lipids, LDs, cytoplasm. | 1 mM (DMSO) | 2 μM | 490/588 - 620 | 2-4 ns68 |
Mito Tracker Green (MTG) | Mitochondria | 10 μM (DMSO) | 100 nM | 490/497 - 551 | 0.5-1.3 ns [this study] |
Mito Tracker Red (MTR) | Mitochondria | 10 μM (DMSO) | 100 nM | 581/594 - 654 | 0.8-1.2 ns [this study] |
TMRM | Mitochondrial polarization | 10 μM (DMSO) | 10 nM | 540/559 - 585 | 1.5-2.5 ns52 |
Phalloidin-Texas Red | F-actin labeling in fixed cells | 66 μM | 0.132 μM | 591/608 - 625 | 3-3.3 ns [this study] |
Phalloidin-Alexa Fluor 546 | F-actin labeling in fixed cells | 66 μM | 0.132 μM | 556/566 - 585 | Intensity |
LysoSensor Green DND-189 | Lysosomes | 1 mM (DMSO) | 2 μM | 448/471 - 535 | Intensity |
IgG conjugated with Alexa Fluor Plus 488 | Secondary antibody | 2 mg /mL | 2 μg / mL | 488/494 - 542 | Intensity |
