Fig. 5: Optogenetic manipulation of Drosophila larvae behaviour.

a Schematic of the inverted microscope setup. b ECL emission spectrum (yellow) and activity spectrum of CsChrimson (black line). c Illustration of the three body points (red, blue, green) tracked in larvae to quantify their behavioural response to ECLD illumination, indicated for animal placed on an ECLD pixel with light on (left) and light off (centre), as well as for animal placed away from the active pixel, i.e., off target (right). d Body angle over time for 10 individual larvae placed on an ECLD pixel for an ATR- control (left) and ATR+ experimental animals, i.e., fed with ATR supplemented food (centre), as well as for off target ATR+ animal (right). Light stimulation and darkness periods are 4 seconds and 6 seconds, respectively. e, f Statistical analyses of body angle and bend events for larvae in experimental and control groups as well as off target group during light “on” and “off” phases. g Response probability in control and experimental groups, highlighting the likelihood of bending in response to ECL light stimulation