Table 1 Antibodies used for the immunohistochemical studies
Antibody | Clone | Type | Source | Dilution |
|---|---|---|---|---|
α-fetoprotein | – | Rabbit, polyclonal | Nichirei, Tokyo, Japan | Ready-to-use |
Glypican-3 | 1G12 | Mouse, monoclonal | BioMosaics, Burlington, VT, USA | 1:100 |
SALL4 | 6E3 | Mouse, monoclonal | Sigma-Aldrich, Saint Louis, MO, USA | 1:2000 |
TTF-1 | 8G7G3/1 | Mouse, monoclonal | Dako, Glostrup, Denmark | 1:200 |
β-catenin | 14/Beta-Catenin | Mouse, monoclonal | BD Biosciences, Franklin Lakes, NJ, USA | 1:200 |
CDX2 | AMT28 | Mouse, monoclonal | Santa Cruz Biotechnology, Dallas, TX, USA | 1:50 |
Chromogranin A | DAK-A3 | Mouse, monoclonal | Dako, Glostrup, Denmark | 1:200 |
Synaptophysin | 27G12 | Mouse, monoclonal | Leica, Newcastle Upon Tyne, UK | 1:200 |
CD56 (NCAM) | 1B6 | Mouse, monoclonal | Nichirei, Tokyo, Japan | Ready-to-use |
MUC2 | Ccp58 | Mouse, monoclonal | Leica, Newcastle Upon Tyne, UK | 1:100 |
Cytokeratin 7 | OV-TL 12/30 | Mouse, monoclonal | Dako, Glostrup, Denmark | 1:50 |
Cytokeratin 20 | Ks20.8 | Mouse, monoclonal | Dako, Glostrup, Denmark | 1:200 |
p40 | – | Rabbit, polyclonal | Nichirei, Tokyo, Japan | Ready-to-use |
