Fig. 4
The process of automated image analysis. One fragment in a liver biopsy specimen of Ishak 6 stage is shown for example. a, b SHG/TPEF microscopy image and reticulin stained image. c Auto-detection of septal area and quantification of septa. Each color represented a septal area, the color inside the septal area represented fibers and different cell region. The quantification of septa includes the whole characteristics of septa region (a), the number and proportions of fibers (b) and cellular regions inside the region of septa (c). Right panel shows magnification of selected septal areas (d, e). In the upper right image, the white arrow indicates the aggregated fibers; black arrowheads indicate the distributed fibers. In the bottom right image, black arrows indicate cellular region
