Fig. 2 | Modern Pathology

Fig. 2

From: Immunohistochemical evaluation of nuclear 5-hydroxymethylcytosine (5-hmC) accurately distinguishes malignant pleural mesothelioma from benign mesothelial proliferations

Fig. 2

a The sarcomatoid component of a biphasic mesothelioma (H&E, original magnification 200×). On CAM5.2/5-hmC double stain (CAM5.2—red chromogen; 5-hmC—brown chromogen), both sarcomatoid tumor cells (b, c) and epithelioid tumor cells (d) express cytoplasmic keratin and show effectively 100% loss of nuclear 5-hmC (original magnification 200×). In a separate case of sarcomatoid mesothelioma (e, f), tumor cells similarly show cytoplasmic staining for keratins, with extensive loss of nuclear 5-hmC (original magnification 200×). In both cases, non-tumor inflammatory and spindle cells provide a positive internal control

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