Fig. 2

p53-binding protein 1 (53BP1) expression in nonalcoholic fatty liver disease (nonalcoholic fatty liver disease) patients. a 53BP1 expression (green) in normal, nonalcoholic fatty liver, and nonalcoholic steatohepatitis liver tissues. The nuclei were visualized with 4ʹ’,6-diamidino-2-phenylindole, dihydrochloride (blue), whereas an antihepatocyte antibody was used to indicate the cell cytoplasm (red). b Quantification of abnormal 53BP1-positive foci (upper panel) and large 53BP1-positive foci (lower panel). Data were calculated as a percentage (%) of the total number of hepatocytes per (n = 9 fields) and are presented as the means ± SEM. *p < 0.01, **p < 0.05. c Coimmunofluorescence of 53BP1 and gamma-histone-2AX (γH2AX). Arrows indicate the coexpression sites. d The number of abnormal foci (upper) and large foci (lower panel) in normal and nonalcoholic fatty liver disease livers with or without the presence of fibrosis. Presence or absence of fibrosis was determined by Azan staining of the specimen. Data are expressed as the means ± SEM. *p < 0.01, **p < 0.05. e The number of abnormal foci (lower left panel) and large foci (lower right panel) in normal and nonalcoholic fatty liver disease livers among the three hepatic zones. Data are expressed as the means ± SEM