Fig. 2

a Endometrial carcinomas show significantly more unstable microsatellite loci than their paired atypical hyperplasia/endometrial intraepithelial neoplasia precursors (p < 0.0001 by paired-samples t-test). All endometrial carcinoma specimens were microsatellite-unstable by next-generation sequencing-based calling (>15% unstable loci, dashed line). Among atypical hyperplasia/endometrial intraepithelial neoplasia specimens, eight showed microsatellite instability, six were microsatellite-stable (<9% unstable loci, dotted line), and three were indeterminate (9–15% unstable loci). b Endometrial carcinomas also show significantly greater tumor mutational burden than paired atypical hyperplasia/endometrial intraepithelial neoplasia precursors (p < 0.0001 by paired-samples t-test). c The difference in tumor mutational burden between atypical hyperplasia/endometrial intraepithelial neoplasia and paired endometrial carcinoma specimens is significantly correlated with the difference in percentage of unstable microsatellites between atypical hyperplasia/endometrial intraepithelial neoplasia and paired carcinoma specimens (p = 0.0002; R² = 0.60). EIN/AH, atypical hyperplasia/endometrial intraepithelial neoplasia. EC, endometrial carcinoma. MB, megabase. TMB, tumor mutational burden. “Δ” indicates an arithmetic difference