Fig. 1

The combination of morphology and patterns of immunolabeling can be used to define key structures in cleared tissues. Desmin immunolabeling of arterial walls (A) is more intense and the media of arteries is thicker than those of muscular veins (V). The concentric wavy autofluorescent lines of the elastic lamina also highlights arterial walls. [Blue labeling for desmin highlights the muscular layer in blood vessels]