Fig. 3: Detection of IDH2 R172 hotspot mutations by immunohistochemistry is feasible in excision and core needle biopsy specimens. | Modern Pathology

Fig. 3: Detection of IDH2 R172 hotspot mutations by immunohistochemistry is feasible in excision and core needle biopsy specimens.

From: Immunohistochemical analysis of IDH2 R172 hotspot mutations in breast papillary neoplasms: applications in the diagnosis of tall cell carcinoma with reverse polarity

Fig. 3

Representative hematoxylin and eosin (a, c) and corresponding IDH2 R172 immunohistochemical expression micrographs of TCCRP30 (a, b) and TCCRP19 (c, d) showing strong and diffuse immunoreactivity for IDH2 R172 (b, d) with a cytoplasmic granular pattern (b–d, insets). Representative (e) hematoxylin and eosin and (f) IDH2 R172 expression photomicrographs of TCCRP20 displaying strong immunolabeling for IDH2 R172; in contrast the adjacent benign breast epithelium shows no immunoreactivity; (g, h) the core needle biopsy specimen of case TCCRP24 shows strong cytoplasmic and granular IDH2 R172 immunoreactivity. Scale bars in a, b 100 μm; c–f 50 μm; g, h 200 μm.

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