Fig. 6: Tumor heterogeneity: spatial and inferred phylogenetic relationship of lesions of patient UC12.

a (Left) The anatomic location of the analyzed samples is depicted along the colon frame. Selected somatic mutations and genome-wide copy number alterations are displayed with the respective histology. The outer circle indicates arm-level chromosomal gains (red) and losses (blue); chromosomes are labeled at the centromere. (Right) Corresponding inferred phylogenetic relationship of analyzed lesions. The different colors in the circles correspond to the color of the lesions on the left part. b Representative images of TP53 immunohistochemistry of carcinoma 1 and 2 with adjacent mucosa, showing a ‘diffuse pattern’ characterized by strongly positive nuclei aggregated in most areas of the crypts, indicating TP53 mutation; remnants of single dysplastic crypts with abnormal TP53 staining indicate emergence of carcinoma 2 from a TP53 aberrant field. c Rectal mucosa with aberrant TP53 staining characterized by a ‘diffuse pattern’ with strongly positive nuclei aggregated in most areas of the crypts, however, without direct contact to carcinoma 1 or 2. CA carcinoma, DYS dysplasia, INF inflamed mucosa.