Fig. 6: FOS-VIM fusion in case 1.
From: Recurrent FOS rearrangement in proliferative fasciitis/proliferative myositis
a RNA sequencing detected a FOS-VIM fusion transcript, which was validated by RT-PCR and Sanger sequencing. The breakpoint in FOS was located at c.724 in the middle of exon 4, which was fused to the reverse complementary strand of exon 1 of VIM. b The predicted protein lost 138 C-terminal amino acids (AA) of wild-type c-FOS protein and gained additional 10 AA at C-terminal end before the stop codon, which maintains the leucine zipper domain (LZD) but loses the transactivation domain (TAD) of c-FOS protein. c The presence of FOS-VIM fusion was also validated by VIM rearrangement as detected by a VIM break-apart FISH assay (arrows indicate split 3′ and 5′ probes).
