Table 3 Primers and sequences for high-resolution melting, pyrosequencing, PCR, and RT-PCR.
From: Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples
Gene | HRM | |
|---|---|---|
IDH1 | IDH1-HRM-F: 5′-TGG ATG GGT AAA ACC TAT CAT CA-3′ IDH1-HRM-R: 5′-GAC TTA CTT GAT CCC CAT AAG CA-3′ | |
IDH2 | IDH2-HRM-F: 5′-AAC ATC CCA CGC CTA GTC CCT-3′ IDH2-HRM-R: 5′-CTC TCC ACC CTG GCC TAC CT-3′ | |
GNAS | GNAS-HRM-F: 5′-TCC ATT GAC CTC AAT TTT GTT TCA G-3′ GNAS-HRM-R: 5′-AAG TTG ACT TTG TCC ACC TGG AAC T-3′ | |
Gene | Pyrosequencing | Sequence to analyze |
|---|---|---|
IDH1 | IDH1-PY-F-bio: 5′-TGG ATG GGT AAA ACC TAT CAT CA-3′ IDH1-PY-R: 5′-GAC TTA CTT GAT CCC CAT AAG CA-3′ IDH1-PY-S: 5′-TGA TCC CCA TAA GCA T-3′ | GACNACCTAT GAHGACCTAT |
IDH2 | IDH2-PY-F: 5′-AAC ATC CCA GCG CTA GTC CCT-3′ IDH2-PY-R-bio: 5′-CTC TCC ACC CTG GCC TAC CT-3′ IDH2-PY-S: 5′-AGC CCA TCA CCA TTG-3′ | GCAGBCACG GCANGCACG |
GNAS | GNAS-PY-F: 5′-TGT TTC AGG ACC TGC TTC G-3′ GNAS-PY-R-bio: 5′-ACC TGG AAC TTG GTC TCA AAG AT-3′ GNAS-PY-S: 5′-AGG ACC TGC TTC GCT-3′ | GCYRTGTCCT |
Gene | PCR | |
|---|---|---|
MDM2 | MDM2-PCR-F: 5′-CCG GAT GAT CGC AGG TG-3′ MDM2-PCR-R: 5′-AAA AGC TGA GTG AAC CTG CCC-3′ | |
ALB | ALB-PCR-F: 5′-TGA AAC ATA CGT TCC CAA AGA GTT T-3′ ALB-PCR-R: 5′-CTC TCC TTC TCA GAA AGT GTG CAT AT-3′ | |
B2M | B2M-PCR-F: 5′-TGA CTT TGT CAC AGC CCA AGA TA-3′ B2M-PCR-R: 5′-AAT CCA AAT GCG GCA TCT TC-3′ | |
