Table 1 Immunohistochemical antibodies and staining protocol.
Antibody | Manufacturer | Clone | Concentration | Pretreatment | Incubation time /detection |
|---|---|---|---|---|---|
BCOR | Santa Cruz Sc-514576 | C-10 | 1/50 | CC2 (56′) | 60′/OptiView universal DAB |
CD99,MIC2 | Dako, Glostrup, Danemark (M3601) | 12E7 | 1/100 | CC1 short (36′) | 32′/UltraView universal DAB |
Pan-Keratin | Roche Diagnostics (760-2595) | (AE1/AE3/PCK26) | Prediluted | CC1 short (36′) + Protéase3(4′) | 8′/UltraView universal DAB |
Desmin | Roche Diagnostics (760-2513) | DE-R-11 | Prediluted | CC1 short (36′) | 32′/ UltraView universal DAB |
ERG | Dako, Glostrup, Danemark (M7314) | EP111 | 1/100 | CC1 short (36′) | 32′/ UltraView universal DAB |
PEA3-ETV4 | Santa Cruz Sc-113 | 16 | 1/25 | CC1 standard (64′) | 32′/OptiView universal DAB + amplification (8’) |
PS100 | Dako, Glostrup, Danemark (Z 311) | Rabbit polyclonal | 1/2000 | CC1 (20′) | 32′/UltraView universal DAB |
WT1-cTer | Zytomed Systems GmbH 14163 Berlin, Germany (523-3994) | Rabbit polyclonal | 1/100 | CC1 standard (64′) | 32′/UltraView universal DAB |
NKX3-1 | Bio SB 93111 Santa Barbara, CA, USA | EP356 (CE/IVD) Rabbit monoclonal | Prediluted | CC1 (48 min) | 36′/OptiView universal DAB |
AGGRECAN | Abcam (ab186414) | EPR14664 | 1/1000 | CC1 standard (64′) | 52′/UltraView universal DAB |
