Fig. 2

Ahnak is expressed in p11-positive and p11-negative neurons and blood vessels in the hippocampus and PFC. a–d Immunohistochemistry reveals that Ahnak is expressed ubiquitously in neurons and blood vessels in the DG (a, b) and PFC (c, d). p11-expressing cells were examined in the ventral DG of BAC transgenic mice expressing p11 promoter-driven EGFP. Confocal microscopy reveals the expression of Ahnak in p11-positive cells in both the DG (a, b) and PFC (c, d). This colocalization is shown at high magnification of the dotted rectangular regions in a and c (b, d). Arrows with filled heads indicate Ahnak/p11 colocalization in vessels; arrows with unfilled heads indicate Ahnak in vessels that are p11-negative. p11/Ahnak colocalization in neurons is indicated by filled arrow heads, while Ahnak in neurons that are p11-negative is indicated by unfilled arrow heads. GC, granule cell layer. Cortical layers are indicated in c. Nissl counterstaining was used to visualize cellular patterns. Scale bars, 100 µm