Fig. 2
a, b Comparison of PRS between FAMBD cases and FAMunaffected. The plots show one-sided p-values, following the hypothesis that BD cases have higher PRS than unaffected individuals. Further details of the plots are as described in the legend for Fig. 1. See Supplementary Fig. S5 and Table S6 for more detailed plots and full association test statistics. c, d Analyses of assortative mating (c) and anticipation (d). These plots were not adjusted for covariates; n = sample size. The y-axis shows the PRS values. c: Assortative mating. The plot shows violin- and boxplots of the BD PRS (pPRS = 0.05), comparing unaffected, married-in individuals with no parent among the ABiF families to other FAM and CC subjects. At pPRS = 0.05, married-in family members showed the highest BD PRS compared to CCcontrols (p = 6.5 × 10−5, Supplementary Fig. S6A and Table S7). The BD PRS of married-in individuals was not significantly higher than the PRS of FAMunaffected at any pPRS (p ≥ 0.167, Supplementary Fig. S6B and Table S7). Covariate used: sex. One-sided p-values were calculated, following the hypothesis that married-in individuals have higher PRS than other unaffected subjects. Note that, in the context of assortative mating, the boxplots of affected BD cases are displayed for reference only and have not been included in the analysis. d Anticipation: the BD PRS did not increase across generations. The plot shows violin- and boxplots of the BD PRS (pPRS = 1 × 10−5) across different generations of the FAM sample for the three diagnosis groups. At pPRS = 1 × 10−5, the association of the BD PRS with generation was strongest but not significant (p = 0.45; Supplementary Fig. S7A and Table S8). Married-in family members were excluded from this analysis. Covariates used: sex, age at the interview, diagnostic group. One-sided p-values were calculated, following the hypothesis that the PRS increase across generations
