Fig. 2

MR deletion disrupts CA2 molecular profile. a MR protein was detectable in CA2 at PN4 in control mice (left panel, NesCre^–), but was not present in CA2 neurons of MR knockout mice (right panel, NesCre⁺ ). b NECAB2, an early marker of CA2 (left panel), was similarly not present in CA2 at PN4 of NesCre⁺ mice (right panel). c RGS14, a robust CA2 marker in adulthood, was not present in CA2 of NesCre⁺ mice older than PN45. DAPI nuclear stain is in blue for all images; scale bars = 100 μm. d Quantification of immunofluorescence data shown in a–c; data are normalized to NesCre^– mice (represented by red dashed line). Full statistics are shown in Supplemental Fig. 1. e NanoString mRNA analysis of hippocampal lysates at PN28 revealed 37 differentially expressed genes in NesCre⁺ compared with NesCre^– mice. All genes shown here differed with an uncorrected p-value of <0.05, and genes that met the adjusted p-value of < 0.05 are designated with *. This analysis confirmed several CA2 markers (green) were depleted after embryonic MR deletion, while several CA1 (purple) and CA3 (orange) markers were significantly higher after embryonic MR deletion. Differences are presented as log₂ fold-change compared with NesCre^– mice. Genes that are abundant in more than one hippocampal region are designated with a plus symbol. f Representative images of MR protein expression in CA2 of Am2Cre^– (left) and Am2Cre⁺ (right) mice (PN28). g Representative image showing that cre was undetectable in CA2 of Am2Cre⁺ adult mice (right panel). The left panel shows cre staining in an Amigo2-cre line without floxed MR (MR fl^–/fl^–). h–j MR (h) and RGS14 (i) were decreased at PN14 after postnatal MR deletion (right panels), but GR staining (j) was increased in area CA2. k Quantification of immunofluorescence stains from PN14 shown in h–j are shown normalized to Am2Cre^– mice (represented by red dashed line). Full statistics are shown in Supplemental Fig. 1. DAPI nuclear stain is in blue for all color images. Scale bars = 100 μm. Refer to Supplemental Fig. 3 for bregma coordinates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001