Fig. 2: Alterations in circHomer1a but not linear HOMER1 mRNA expression in SCZ DLPFC and stem cell-derived neuronal cultures of subjects with SCZ and BD.
From: A psychiatric disease-related circular RNA controls synaptic gene expression and cognition
Mean ± SEM relative to Control circHomer1a (a) and circCUL4A (b) levels (qRT-PCR, normalized to the geometric mean of highly expressed and unaltered circTulp4 and CDR1as; see also Supplementary Fig. 3a) in the DLPFC of subjects with BD and SCZ. c Relative to Control changes in circHomer1a in the DLPFC of patients with SCZ are positively correlated to the age of onset of the disease. Spearman correlation coefficient and two-tailed p values are shown in the graph. d Mean ± SEM relative to Control HOMER1 mRNA levels (qRT-PCR, normalized to the highly expressed and unaltered 18S rRNA; see also Supplementary Fig. 4c) in the DLPFC of subjects with BD and SCZ. a, b, d **p < 0.01, based on a Univariate General Linear Model corrected for RIN, PMI, RI, and brain pH. In all postmortem data graphs, individual SCZ (red circles), BD (green circles), and Control (blue circles) sample values are shown. Mean ± SEM relative to the mean of Control neuronal progenitors (NPs) circHomer1a (e) and HOMER1 mRNA (f) levels in iPS cell-derived SCZ patient and Control (n = 10 Control and 9 SCZ subjects) NPs and 6-week differentiated neurons. Mean ± SEM relative to the mean of Control NPs circHomer1a (g), and HOMER1 mRNA (h) levels in iPS cell-derived BD patient and Control (n = 3 Control and 4 BD) NPs and 2-, 4-, and 6-week differentiated neurons. e–h #0.10 > p > 0.05, *p < 0.05, two-tailed one sample t-test relative to the Control of the same developmental time-point. In all bar graphs the individual replicates are shown within the graph.
