Fig. 1: Cellular landscape of basal TSPO expression in the hippocampus of adult mice.

a Visualization of single-cell RNA-sequencing (scRNA-seq) data using t-distributed stochastic neighbor embedding (tSNE), showing the clustering of 9 main cell populations. Cellular sub-clusters are provided in Fig. S3 (Supplementary Information). Corresponding uniform manifold approximation and projection (UMAP) scores are shown in Figure S2 (Supplementary Information). b TSPO expression (in red) in individual clusters of cells as detected by scRNA-seq. c Percentage of cells expressing TSPO in each of the 9 main cell populations, as measured by scRNA-seq. d tSNE scores of neuronal sub-clusters (neuronal clusters 1–4) and percentage of cells expressing TSPO in each of the 4 neuronal sub-clusters. e Differential gene expression (indexed as average log2 fold change, Log2FC) between neuronal cluster 3 and neuronal cluster 1/2/4, showing differentially expressed genes included in the eukaryotic initiation factor 2 (EIF2) signaling pathway and oxidative phosphorylation as identified by Ingenuity Pathway Analysis (IPA). The significance of differential gene expression is given as –Log10(p-value).