Fig. 2: Immunohistochemical localization of TSPO protein in neuronal and non-neuronal hippocampal cells of adult mice using immunofluorescence staining analyzed by confocal laser scanning microscopy.
From: Neuronal activity increases translocator protein (TSPO) levels
The photomicrographs show representative Z-stack images acquired through confocal microscopy, with nuclear staining (DAPI) in blue, TSPO in green and various CNS cells of interest in red. TSPO co-localizing with the cellular markers of interest appears in yellow. Examples of co-localization areas are highlighted by the crosshair. a Examples of neuronal TSPO protein expression, as evaluated using the post-mitotic neuronal markers NeuN, SMI-32 and MAP-2. b Examples of non-neuronal TSPO protein expression, including expression in Iba1-positive microglia, GFAP-positive astrocytes and Glut1-positive vascular endothelial cells.
