Fig. 1: Psen1KI/KI alters microglial morphology and function in vivo.
From: Presenilin 1 phosphorylation regulates amyloid-β degradation by microglia
a Representative time-lapse response of microglia to laser injury in vivo. Volume stacks of microglia in the CX3CR1-EGFP mice are visualized through a cranial window with multi photon imaging immediately following a focused laser injury (indicated by a dashed white circle). Scale bar represent 10 µm b Time profile of microglia branch encroachment into injury region. c Representative 3D filament tracings of entire microglia in the cortex of WT and Psen1KI/KI mouse brains after Iba1 labeling and high-resolution confocal stack imaging. Scale bars represent 10 µm d Characterization of microglia branching: total branch length, total branch volume, total number of segments (between junction points), segment length, segment volume, number of terminal branches. Total volume refers to total volume of all branches of single microglia, it does not include cell body (schematic diagram Supplementary Fig. 1c). e Average length, volume, cross sectional area and frequency of segments at the given branch depth from the soma. Brown gradients represent points where statistical significance was observed. Bar and line plots represent means ± sem. *p < 0.05, two sample t test. For in vivo experiments, N = 5–7 mice per group. For microglia morphology, N = 10 microglia per mouse, three different mice for each genotype. Blind analyses were performed.
