Fig. 3: Development of a novel co-grafting procedure for both hBChE and GLP1 epidermal stem cells. | Molecular Psychiatry

Fig. 3: Development of a novel co-grafting procedure for both hBChE and GLP1 epidermal stem cells.

From: Reducing alcohol and/or cocaine-induced reward and toxicity via an epidermal stem cell-based gene delivery platform

Fig. 3

a hBChE and GLP1 co-grafted skins. Sections of grafted skin were immunostained with various antibodies and were visualized. (Krt14: keratin 14 is a basal stem cell marker for skin epidermis; Krt10: keratin 10 is a marker for early epidermal differentiation, Lor: Loricrin is a marker for early epidermal differentiation, β4: β4-integrin, CD104 is a marker for skin basement membrane). Dashed lines represent the skin basement. Epi epidermis, Der dermis. Scale bar = 50 μm. b, c Levels of hBChE and GLP1 in the co-grafted mice. The presence of hBChE (b) and GLP1 (c) in blood was quantified by ELISA (n = 3 mice per group). GLP1 expression was induced by doxycycline (Dox) diet in GhBChE/GLP1 mice.

Back to article page