Fig. 2: Cul3+/− mice have altered brain morphology.
a Voxel-wise analysis highlighting significant differences in relative volumes throughout the brain between WT and Cul3+/− mice with 5% false discovery rate (FDR). Left panel is absolute changes and right panel is relative change in brain regions. Scale bar 2.7–10.7 indicates decreasing FDR, where 2.7 = 5% FDR. Red color signifies increased and blue color signifies decreased brain volume compared with WT brain. b Cul3+/− mice have reduced absolute brain volume (**p < 0.01) compared to WT mice (WT n = 18, Cul3+/− n = 20). c Reduced relative grey matter volume in Cul3+/− mouse brain (*p < 0.05). d No significance difference is observed in relative white matter volume. e MRI revealed significant reduction in relative volume, normalized by total brain volume, of primary somatosensory cortex (*p < 0.05); hippocampal DG region (***p < 0.001); cerebellum (**p < 0.01). Dots represent individual animals; ○-female and ∆-male. f Reduced cortical thickness is observed by MRI in Cul3+/− mice, turquoise color is decreased, red/orange color is increased. g Reduced cortical thickness of somatosensory cortex in adult Cul3+/− mice, left panel shows somatosensory cortex region stained with NeuN (mature neuron marker) and BRN2 (layer II–IV marker); Scale bar is 100 μm. h Reduction of total cortical thickness, layer thickness and density of NeuN- and BRN2-positive cells/area in layer II–IV are observed (*p < 0.05; two-tailed t-test, n = 6 for each genotype). Dots represent independent samples; two-tailed t-test used for (b–h); error bars represent mean ± SD.
