Fig. 4: Lactate, β-hydroxybutyrate, and NADH suppress ROS production induced by corticosterone in adult hippocampal stem/progenitor cells in vitro.
From: Role of adult hippocampal neurogenesis in the antidepressant actions of lactate
Analysis of ROS production. Cultures of adult hippocampal stem/progenitor cells were treated for 48 h with corticosterone (5 μM) together with either lactate (20 mM) (a), pyruvate (20 mM) (a), β-hydroxybutyrate (20 mM) (b), acetoacetate (20 mM) (b) or NADH (100 μM) (c). Left panels: histograms of CellROX intensity per cell shown as the percentage of the vehicle. Right panels: illustrations of CellROX fluorescence (green) in stem/progenitor cells counterstained with NucBlue (Hoechst)(blue). Data are the mean ± SEM. One-way ANOVA followed by Tukey post-hoc test (n > 17/condition). *p < 0.05. NS not significant.
