Table 1 Antibodies used.
Reagent or resource | Analysis | Dilution | Company | Cat. no. |
|---|---|---|---|---|
NR2a | WB | 1:1000 | Merck | AB10531 |
NR2b | WB | 1:500 | NeuroMab UC Davis | 75-097 |
Phospho-NR2b phospho-Y-1472 | WB | 1:500 | Sigma | M2442 |
ERK | WB | 1:1000 | Cell Signaling Technology | #4696 |
Phospho-ERK 1/2 | WB | 1:1000 | Cell Signaling Technology | 4370 |
S6 | WB | 1:1000 | Cell Signaling Technology | #2317 |
Phospho-S6 | WB | 1:1000 | Cell Signaling Technology | #4858 |
PSD95 | WB | 1:1000 | Merck Millipore | MABN68 |
Actin | WB | 1:2000 | Merck Millipore | MAB1501R |
Glt-1 (EAAT1) | IHC | 1:1000 | ThermoFisher | PA5-17099 |
TRPA1 | WB | 1:500 | Merck Millipore | ABN1009 |
GAP43 | WB | 1:1000 | Merck Millipore | MAB347 |
Serine racemase (D5V9Z) | WB | 1:500 | Cell Signaling Technology | #29798 |
GLAST (EAAT2) | IHC | 1:1000 | ThermoFisher | PA5-19709 |
GFAP | IHC | 1:2000 | Synaptic Systems | 173004 |
DCX | IHC | 1:500 | Cell Signaling Technology | 4604S |
WFA-biotin | IHC | 1:2500 | Vector Labs | B1355 |
HABP-biotin | IHC | 1:400 | AMSBIO | AMS.HKD-BC41 |
GFAP | IHC | 1:1000 | Abcam | ab4674 |
Iba1 | IHC | 1:1000 | Wako Japan | #019-19741 |
Goat anti-chicken-488 | IHC | 1:1000 | Invitrogen | A-11039 |
Goat anti-rabbit-647 | IHC | 1:1000 | Invitrogen | A-32733 |
Anti-mouse IRDye800CW | WB | 1:10,000 | LiCor | 926-32210 |
Anti-rabbit IRDye680RD | WB | 1:10,000 | LiCor | 926-68070 |
