Fig. 3: Analysis of hippocampal synapse-enriched protein fractions of SH-WT and SH-RX mice revealed line-specific alterations in pre- and postsynaptic pathways.

A Based on the abundant proteins of hippocampal synapses, the cellular component analysis of ConsensusPathDB database proposed that the synaptic membrane, neuron part, postsynaptic density, cell projections, and neuronal spines might be affected in SH-WT and SH-RX mice. B The biological process analysis proposed significant alterations in the behavior, dendritic organization, and altered vocalization for SH-WT mice but not for SH-RX mice. C The molecular function analysis predicted alterations in the scaffold organization of glutamatergic synapses for SH-WT mice and alteration in the semaphorin receptor function for axon guidance in SH-RX mice. D The KEGG pathway analysis suggested that the glutamatergic synapses are affected in SH-WT, whereas the axon guidance pathway is most likely impaired in SH-RX mice. A–C ConsensusPathDB identified on level 2, are given in the yellow field and were eventually more specified in the third (pink) and fourth level (green). LTP = Long-term potentiation; ALS = Amyotrophic lateral sclerosis.