Fig. 4: Behavioral analysis of SH-WT and SH-RX mice revealed reversible social alterations.

A Behavioral experiments of adult SH-WT mice revealed increased repetitive rearing and normal self-grooming in the LABORAS test, a borderline significant decrease in contacts in the novel object recognition test, a normal number of USV calls, nesting impairment, reduced direct social interaction, and reduced number of contacts in the third session of the three-chamber social test (n = 12 SH-WT and 15 littermate control mice, 6–8 months). B Behavioral experiments of adult SH-WT^P10-on mice revealed a slight increase in repetitive rearing but no social impairment in the novel object recognition, nesting, direct social interaction, or three-chamber social tests (n = 14 SH-WT^P10-on and 16 and Ctrl.^P0-on mice, 4–6 months). C Behavioral experiments of adult SH-WT^Ad-off revealed increased repetitive rearing and reduced nesting behaviors but no social impairment in the novel object recognition, direct social interaction, or three-chamber social tests (n = 12 SH-WT^Ad-off and 15 Ctrl.^Ad-off mice, 5-8 months). D Behavioral analysis of adult SH-RX mice revealed normal rearing and reduced repetitive self-grooming in the LABORAS test with an enhancement of social interaction in the novel object recognition, USV, and direct social interaction tests. The nesting and three-chamber social test results were comparable to control mice (n = 10 SH-RX and 16 littermate control mice, 6–8 months). E Behavioral analysis of adult SH-RX^Ad-off revealed a significantly reduced number of self-grooming but normal social interaction in the novel object recognition, nesting, USV, direct social interaction, and three-chamber social tests (n = 9 SH-RX^Ad-off and 14 Ctrl.^Ad-off mice, 5–8 months). For all experiments, Two-way ANOVA was used (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Error bars indicate the standard error of the mean (SEM).