Fig. 3: Aged mice lose C6S. Knockout of C6 sulfotransferase levels lead to premature memory loss.
From: Chondroitin 6-sulphate is required for neuroplasticity and memory in ageing
A Increase in the ratio of C4S/C6S in aged mice suggests a depletion of C6S. Left, general interstitial matrix. Right, PNN matrix. B OR memory loss (3 h) in chst3 knockout mice. Unpaired two-tailed t-test. 11 weeks: *p = 0.0317. 13 weeks: *p = 0.0306. 18 weeks: *p = 0.0165. C Impaired spontaneous alternation performance in chst3 knockout mice. Unpaired two-tailed t-test, 4 M: **p = 0.0011. 7 M: **p = 0.001. D Atypical marble-burying behaviour in chst3 KO mice on ageing. Unpaired two-tailed t-test. 12 M: **p < 0.01. In WT the ageing impaired marble-burying behaviour, 7 M vs 12 M ***p < 0.001. E Experimental timeline of AAV-chst3 injection to chst3 KO mice at 4 M. F chst3 gene delivery recovered memory in 4 M chst3 KO mice line. Unpaired two-tailed t-test, 3 h delay: **p < 0.01.
