Fig. 4: ARMI is prevented in animals overexpressing C6 sulfotransferase. Restoration of C6S restores LTP in brain slices.
From: Chondroitin 6-sulphate is required for neuroplasticity and memory in ageing
A Experimental timeline of AAV-chst3 injection to 5 M or 19 M C57BL/6 mice. B Age-mediated memory loss in C57bl/6 mice and SOR memory restoration by injection of AAV1-Chst3 to PRh. Three hours memory retention: *p = 0.023. Six hours memory retention: **p = 0.0071. In young mice (6 M), 24 h memory was enhanced after AAV-chst3 injection. *p = 0.0317. Data present as mean ± SEM. All data were analysed by unpaired two-tailed t-test. C In chst3 transgenic mice, age-mediated memory loss was absent. Age: 20 M, 6 h memory retention: WT vs Tg *p = 0.0361. Data present as mean ± SEM. D LTP in perirhinal cortex slices in chst3 KO mice. Left: LTP is significantly decreased (two-way RM ANOVA: *p = 0.018). Left inset: examples of fEPSP. Right: I/O curve of evoked fEPSP amplitude (two-way RM ANOVA, n.s. p = 0.758). E LTP is increased in CA3-CA1 in chst3 KO mice (two-way RM ANOVA, *p = 0.010). Left inset: Examples of fEPSP. Right: I/O curve (two-way RM ANOVA, n.s. p = 0.457). Graphs are mean ± SEM. F LTP in perirhinal cortex in AAV-chst3 injected aged mice. (a) Timeline. (b) LTP in the perirhinal cortex (two-way RM ANOVA: *p = 0.036). Left inset: examples of fEPSP. (c) I/O curve (two-way RM ANOVA, p > 0.05).
