Fig. 1: DREADD activation of septal OXTr neurons.
From: Activation of septal OXTr neurons induces anxiety- but not depressive-like behaviors
a A representative image of hM3Dq-mCherry-transduced OXTr neurons in the septum and their projections to the HDB. b, c J60 treatment via i.p. injections potently increased Fos positive OXTr neurons in the hM3Dq-mCherry transduced mice (n = 5) compared to mCherry-transduced mice (n = 3): (b) Representative images of Fos and hM3Dq-mCherry in the septal OXTr neurons of hM3Dq-mCherry transduced animals; (c) Quantification of the percentage of Fos positive OXTr neurons transduced with hM3Dq-mCherry (hM3Dq, n = 5; mCherry; n = 3; p = 0.0002; Unpaired student t test). d–f Loose-seal cell-attached recordings: adding J60 to the circulating aCSF increased the firing rates at the (d) hM3Dq-transduced OXTr neurons but not the (e) mCherry-transduced neurons in acute brain slices of the virally transduced Oxtr-Cre mice; (f) Group data of action potential firing rates relative to baseline in hM3Dq- hM4Di-, or mCherry-transduced OXTr neurons with J60 (n = 5 per group). One-way ANOVA was used for (f), and an unpaired student t-test for (c). Mean ± SEM; **p < 0.01; ***p < 0.001. Scale bars, 2 mm for (a); and 50 µm for (b).
