Fig. 5: Regulation of the cell cycle and the re-location of the microtubule-organizing center by the circTshz2-2/TrkB signaling pathway.

A Changes in the cell cycle in circTshz2-2-depleted differentiated Neuro-2A cells at day 5 following treatment with a TrkB antagonist (ANA-12) and p75NTR inhibitor (PD90780). The data represent three independent repeats (n = 3) and are expressed as the mean ± SEM. Percentages of cells in each phase were calculated using FlowJo software. siCtr indicates the negative control siRNA. PE-A indicates phycoerythrin-area. 2N, 2N–4N, 4N, <2N, and >4N indicate the phase of G1, S, G2/M, sub-G1, and phases greater than G2/M, respectively. Statistical significance was analyzed using an unpaired two-tailed t-test with Welch’s correction; ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001. B The changes in the nuclear morphology and the re-location of the microtubule-organizing center following circTshz2-2 knockdown in differentiated Neuro-2A cells at day 5. The beta-tubulin, gamma-tubulin (triangle), and nuclei (arrow) are indicated by red, green, and blue, respectively. The data are shown as representative cells from three independent cultures (n = 3). In A and B, the sicircTshz2-2 data are derived from cells treated with a mix of two different siRNAs against circTshz2-2 (#1 and #2).