Fig. 6: Regulation of spatial memory by circTshz2-2 in wild-type and obese mice.

A Illustration displaying the infusion location of brain infusion kit and osmotic pump on the brain. The infused position at the lateral ventricle is mediolateral 1.0 mm from bregma, anteroposterior 0.3 mm, and dorsoventral 2.5 mm, in the order. B Illustration displaying the implantation of osmotic pump containing a solution (control siRNA or circTshz2-2 siRNAs). The brain infusion kit was inserted into the lateral ventricle. The osmotic pump was fixed in the dorsal subcutaneous area. sicircTshz2-2 (#1 + #2) indicates a mixture of two independent circTshz2-2 siRNAs. C The expression of circTshz2-2 in the brain cortex and hippocampus of wild-type (WT) and transgenic obese (ob/ob) mice, respectively, is described as the mean ± SEM (n = 5). D Changes in the expression of circTshz2-2 following circTshz2-2 depletion in the brain cortex and hippocampus of wild-type mice are described as the mean ± SEM (n = 5). E Changes in the expression of circTshz2-2 following circTshz2-2 depletion in the brain cortex and hippocampus of obese mice are described as the mean ± SEM (n = 5). F Illustration describing the movement of wild-type and obese mice in the T-maze apparatus after infusion of either control siRNA or circTshz2-2 siRNA mixtures. The representative trace indicates the movement for 5 min of five mice per group. G Changes in the percentage of spontaneous alternation following circTshz2-2 depletion in the wild-type and obese mice are described as the mean ± SEM (n = 5). It displays the average percentage of spontaneous alternation obtained from days 1 and 3 among the trials. The dots on the bar mean a percentage of spontaneous alternation of the mice in each group. H Changes in the percentage of spontaneous alternation at days 1 and 3 following circTshz2-2 depletion in wild-type and obese mice are described as the mean ± SEM (n = 5). I Changes in the level of Cyclin B2 and CDK1 following circTshz2-2 depletion in the brain cortex and hippocampus of wild-type and obese mice are represented as the mean ± SEM (n = 5). In D–I, WT-siCtr indicates the control siRNA-infused wild-type mice. WT-sicircTshz2-2 (#1 + #2) indicates the wild-type mice infused with the mixture of two independent circTshz2-2 siRNAs. ob/ob-siCtr indicates control siRNA-infused obese mice. ob/ob-sicircTshz2-2 (#1 + #2) indicates the obese mice infused with the mixture of two independent circTshz2-2 siRNAs. In C–E, and G–I, statistical significance was determined using an unpaired two-tailed t-test with Welch’s correction; ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001.