Fig. 4: Dopamine 1 receptors in MDNCs from CTL and SCZ.

A Flow cytometric diagram showing expression of dopamine 1 receptors (D1R) in monocytes. Gray histograms represent control isotypic labeling. White histograms represent specific labeling. B Flow cytometric diagrams showing expression of D1R in MDNCs from a healthy control and a patient with SCZ. Gray histograms represent control isotypic labeling. White histograms represent specific labeling. C Dot plots contrasting expression of D1R in MDNCs from CTL vs. SCZ (CTL, 46.4 ± 6.7; SCZ, 31.3 ± 5.2; P = 0.15) and CTL vs. MED (CTL, 46.4 ± 6.7; MED, 24.2 ± 3.4; P = 0.03). D1R expression was measured via flow cytometry. Non-parametric Mann–Whitney test was used to make pairwise comparisons between groups. Data are given as mean ± SEM. Cells from 7 CTL, 7 SCZ and 5 MED were included in the analysis. *P = or < 0.05. D Bar graphs showing the effects of SCH-23390, a D1R antagonist, in dopamine-elicited pruning in MDNCs. Dopamine was used at 4 mM. For LPN, dopamine, 63.5 ± 2.2%; SCH, 54.7 ± 2.8%; P = 0.0093; LSN, dopamine, 78.9 ± 4.0%; SCH, 87.3 ± 2.3%; P = 0.93; number of secondary neurites, dopamine, 3.6 ± 0.21; SCH, 2.8 ± 0.21; P = 0.08 and total number of neurites, dopamine, 4.0 ± 0.24; SCH, 3.1 ± 0.22; P = 0.08. The non-parametric Mann–Whitney test was used to make pairwise comparisons between groups. Data are given as mean ± SEM. MDNCs from three individuals were included in the analysis. One individual was an unmedicated patient and one healthy subject was tested by duplicate. MDNCs traced for LSN treated with dopamine n = 200; and with SCH-23390 + dopamine, n = 117; for all other structural parameters; treated with dopamine n = 221; and with SCH-23390 + dopamine, n = 125. **P = 0.009.