Fig. 1: Flowchart depicting the steps involved in the creation of the corticolimbic DCC-ePRS score.
From: Corticolimbic DCC gene co-expression networks as predictors of impulsivity in children
A The GeneNetwork database was used to generate a Dcc gene co-expression matrix in the PFC and NAcc in mice. Genes with a correlation of co-expression \(\ge\)|0.5| were retained. Brainspan was used to identify human homologous transcripts and to filter each gene list by selecting the transcripts enriched during the first 18 months of life, as compared to adulthood, defined by a differential expression \(\ge\)1.5, within the same brain area. Each resulting gene list comprised the DCC co-expression network for their respective brain area. B Based on their annotation in the NCBI library, using GRCh37.p13 assembly, common SNPs within each co-expression network, GTEx data base, and genotyping cohort were subjected to linkage disequilibrium clumping to remove highly correlated SNPs (r2 \(\ge\) 0.2). Using data from the GTEx project, alleles at a given cis-SNP were weighted by the estimated brain-region-specific effect of the genotype on gene expression. The sum of these estimated effects resulted in ePRS scores for the DCC co-expression networks in the PFC and NAcc, which we aggregated into a single global ePRS score.
