Fig. 1: Study workflow.
From: Brain integrity is altered by hepatic APOE ε4 in humanized-liver mice
A Schematic illustration of the differential fractionation protocol employed for the preparation NE, SE and SD fractions of the dissected brain areas obtained from FRGN humanized-liver and APOE TR mice. B Validation of tissue fractionation efficiency. Lamin B1 was detected only in the NE fraction, while PSD95 was present only in the NE and SE fractions. Synaptobrevin isoforms 1 and 2 (VAMP1/2) was present in all the fractions. C Schematic experimental layout. The right brain hemispheres from n = 18 FRGN (whereof APOΕ ε2/ε3 n = 7 and APOE ε4/ε4 n = 11) and n = 6 TR (whereof APOΕ ε3 n = 3 and APOE ε4 n = 3) mice were utilized. The cortex and hippocampus were dissected from the right hemispheres of n = 6 TR mice (whereof APOΕ ε3 n = 3 and APOE ε4 n = 3) and n = 12 FRGN humanized-liver mice (whereof APOΕ ε2/ε3 n = 4 and APOE ε4/ε4 n = 8). Thalamus and cerebellum were dissected from the right hemispheres of n = 6 FRGN humanized-liver mice, (whereof APOΕ ε2/ε3 n = 3 and APOE ε4/ε4 n = 3).
