Fig. 2: Plasma human apoE levels and endogenous mouse apoE in the FRGN mouse brain.
From: Brain integrity is altered by hepatic APOE ε4 in humanized-liver mice
A Plasma human apoE levels, assessed by ELISA APOE ε2/ε3 (n = 4) versus APOE ε4/ε4 (n = 10) FRGN humanized mice (p = 0.525, assessed by Wilcoxon signed-rank test). B Western blot image showing apoE immunoreactive bands in the NE, SE and SD cortical fractions. C Levels of brain apoE in the SD fraction prepared from thalamus (Th.), cortex (Ctx.), cerebellum (Cb.), and hippocampus (Hipp.) of APOE ε4/ε4 and APOE ε2/ε3 humanized-liver mice. Protein levels were normalized against synaptobrevin isoforms 1 and 2 (VAMP1/2). D Densitometric analyses of immunoreactive bands corresponding to endogenous mouse apoE after normalization against synaptobrevin isoforms 1 and 2 (VAMP1/2) in the SD fraction isolated from the cortex, hippocampus, thalamus and cerebellum from APOE ε2/ε3 and APOE ε4/ε4 FRGN humanized-liver mice. Data is shown as mean or median (minimum–maximum). Group comparisons were done using the Student’s t test (C, D), or Wilcoxon signed-rank test (A). See also Supplementary Fig. 1.
