Fig. 2: Mitochondrial bioenergetics.
A Mitochondrial membrane potential of NPCs. Red/green (JC-1 aggregate/monomer) ratios of control (CNTRL) and MDD NPCs. Dot plots show mean red/green ratios ± SEM, control n = 8, MDD n = 8. Difference is not significant. B, C Oxygen consumption rates (OCR) of control and MDD NPCs. B Representative OCR measurement during the Mito Stress Test for MDD and control NPCs. C Maximal respiration is significantly different as analyzed by two-tailed t-test (indicated by *). Considering our hypothesis of reduced oxygen consumption in MDD cells, a one-tailed t-test indicate significant difference of all parameters except spare-respiratory capacity (indicated by #). Dot plots show normalized mean OCR values ± SEM, Control n = 8, MDD n = 8. D Basal Ca2+ calcium levels were significantly altered in MDD NPCs. Shown are the Fura-2 340 nm/380 nm fluorescence ratios of non-depressed control and MDD NPC lines. Dots show mean ratios (340 nm/380 nm, ratios ± SEM). Control n = 8, MDD n = 8. E ATP levels were not significantly different between MDD and CNTRL NPCs. Dots show normalized mean RLU values ± SEM. F MDD NPCs are significantly smaller than CNTRLs. Size is analyzed by assessing area (pixels) of Fura-2-loaded cells. Significant differences between MDD and non-depressive controls are indicated with *.
