Fig. 4: Electrophysiological characterization of iPSC-derived neurons. | Molecular Psychiatry

Fig. 4: Electrophysiological characterization of iPSC-derived neurons.

From: Induced neural progenitor cells and iPS-neurons from major depressive disorder patients show altered bioenergetics and electrophysiological properties

Fig. 4: Electrophysiological characterization of iPSC-derived neurons.

A Exemplary differential interference contrast photomicrograph (400Ă—) of a cultured iPSC-derived neuron (DIV 21) from a MDD patient. A patch-pipette is attached to the soma of the cell. B Whole-cell recording in the current-clamp mode allows recording of the membrane potential. Injection of a supra-threshold depolarizing current induced action potentials. C Whole-cell recording in the voltage-clamp mode showed voltage-activated transient Na+-inward (INa+) and delayed K+-outward currents (IK+) following a pulse protocol stepping from Vhold = −80 mV to more depolarized potentials (−70 to +40 mV). An exemplary current/voltage relationship for voltage-activated Na+ (INa+, dark gray filled circles) and K+ currents (IK+, light gray filled circles) is presented in (D).

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