Fig. 3: Effect of SAFit2 on hippocampal NPC proliferation.
From: Inhibition of FKBP51 induces stress resilience and alters hippocampal neurogenesis
Hippocampal NPC were isolated from P28 male rats, grown for four days as neurospheres, then dissociated and kept in a proliferative environment with 0.02% BrdU for 4 hs while exposed to 1, 10 or 100 nM SAFit2 or DMSO (A). Representative images of BrdU staining (B). Quantification of nuclear BrdU-positive NPCs revealed that 100 nM SAFit2 significantly increased NPC proliferation (C). Results correspond to three independent cultures with triplicates in each (mean + SEM, n = 9 per group). NPC neuroprecursor cells, DIV day in vitro, P postnatal day. *p < 0.05 vs DMSO, by one-way ANOVA with Tukey post hoc test.
