Fig. 3: MAD1 regulates neurite outgrowth. | Molecular Psychiatry

Fig. 3: MAD1 regulates neurite outgrowth.

From: Schizophrenia-associated Mitotic Arrest Deficient-1 (MAD1) regulates the polarity of migrating neurons in the developing neocortex

Fig. 3

a, b Mouse embryos were electroporated with shCTL or shMAD1 at E14.5 and analyzed at P7. Images were acquired by z-stacking and 3D projection by confocal microscopy. Only EGFP-positive neurons were analyzed, excluding neurons having fragmented structures inevitably damaged during the tissue sectioning. Representative images from each group with traced diagrams (a) and statistical analysis (b, shCTL, n = 50 cells; shMAD1, n = 62 cells) of the apical dendrite length are shown. Dashed lines demarcate pia mater (a). c–g Primary cultured mouse neurons were transfected at 1 day in vitro (DIV1) and analyzed at DIV3. Representative images from each group (c) and statistical analysis of total neurite length (d), longest neurite length (e), and the number of neurites (f, shCTL, n = 47 cells; shMAD1, n = 50 cells; c-MAD1, n = 50 cells; shMAD1 + c-MAD1, n = 50 cells). Sholl analysis of neurite outgrowth (g, shCTL, n = 30 cells; shMAD1, n = 40 cells; c-MAD1, n = 32 cells; shMAD1 + c-MAD1, n = 34 cells). Scale bars represent 100 μm (a) and 50 μm (c), respectively. Student’s t-test was used for statistical significance (b). One-way ANOVA (d–f) and two-way ANOVA (g) followed by Turkey’s post-hoc test were used. Analysis with nested model was conducted (b and d–f; Supplementary Dataset). Data are presented as means ± SEM. Statistical significance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 or n.s. (not significant).

Back to article page